Hotspot

accD

Genome · 58110-59594 · 1485 bp

Back to Meristotropis

Pi 0.00875

MarkerSeek score 63.80

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 1485
variable_sites: 13
pi: 0.00875
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGTCATGTCATGTAAATAGAGGA	AGTTTGCTCTTGTGTGTTCT	34.8	40.0	54.9	55.4	1596-1599 bp	1.0	40.7
accD_p2	TCATCAATTGTCATGTCATGT	AGTTTGCTCTTGTGTGTTCT	33.3	40.0	53.5	55.4	1604-1607 bp	1.0	40.6
accD_p3	TTCATCAATTGTCATGTCATGT	AGTTTGCTCTTGTGTGTTCT	31.8	40.0	54.4	55.4	1605-1608 bp	1.0	40.6
accD_p4	ATTCATCAATTGTCATGTCATGT	AGTTTGCTCTTGTGTGTTCT	30.4	40.0	54.9	55.4	1606-1609 bp	1.0	40.6
accD_p5	TGACTATTCATCAATTGTCATGTCA	AGTTTGCTCTTGTGTGTTCT	32.0	40.0	56.8	55.4	1611-1614 bp	1.0	40.6