Hotspot

trnL-UAG-rpl32

Genome · 123558-124234 · 677 bp

Back to Meristotropis

Pi 0.0225

MarkerSeek score 60.70

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: Genome
length_bp: 677
variable_sites: 14
pi: 0.0225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TCCTAAGAGCAGCGTGTCTA	GGGATATCGGACAGCATTGA	50.0	50.0	57.9	57.2	757-813 bp	1.0	78.0
trnL-UAG-rpl32_p2	GAAGTAGCAAAGCCTTGGGT	GGGATATCGGACAGCATTGA	50.0	50.0	58.1	57.2	1261-1317 bp	1.0	77.2
trnL-UAG-rpl32_p3	GAAGTAGCAAAGCCTTGGGT	AGGGATATCGGACAGCATTG	50.0	50.0	58.1	57.2	1262-1318 bp	1.0	77.1
trnL-UAG-rpl32_p4	ATGGGATTGTAGAGCGGGTA	GGGATATCGGACAGCATTGA	50.0	50.0	57.9	57.2	1319-1375 bp	1.0	76.9
trnL-UAG-rpl32_p5	ATGGGATTGTAGAGCGGGTA	AGGGATATCGGACAGCATTG	50.0	50.0	57.9	57.2	1320-1376 bp	1.0	76.8