Hotspot

ycf1-rps15

Genome · 113440-113983 · 544 bp

Back to Meristotropis

Pi 0.0187

MarkerSeek score 68.50

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: Genome
length_bp: 544
variable_sites: 10
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	ACCCATAATGGAAGCCGTTT	TCAACTGATCCGGGGTTTTC	45.0	50.0	57.5	57.8	678-716 bp	1.0	76.3
ycf1-rps15_p2	ACCCATAATGGAAGCCGTTT	CAACTGATCCGGGGTTTTCT	45.0	50.0	57.5	57.8	677-715 bp	1.0	76.3
ycf1-rps15_p3	ACCCATAATGGAAGCCGTTT	ACTGATCCGGGGTTTTCTTC	45.0	50.0	57.5	57.5	675-713 bp	1.0	73.9
ycf1-rps15_p4	ACCCATAATGGAAGCCGTTT	TTCAACTGATCCGGGGTTTT	45.0	45.0	57.5	57.3	679-717 bp	1.0	72.5
ycf1-rps15_p5	ACCCATAATGGAAGCCGTTT	CTGATCCGGGGTTTTCTTCT	45.0	50.0	57.5	57.2	674-712 bp	1.0	71.6