Hotspot

trnR-ACG-trnN-GUU

Genome · 106928-107472 · 545 bp

Back to Meristotropis

Pi 0.032

MarkerSeek score 70.00

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: Genome
length_bp: 545
variable_sites: 17
pi: 0.032
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCAAGTCCT	55.0	50.0	57.8	58.0	625-637 bp	1.0	82.0
trnR-ACG-trnN-GUU_p2	TGGGAAACAGATCCAGTGGA	TGGTCGTAGGTTCAAGTCCT	50.0	50.0	58.3	58.0	666-678 bp	1.0	81.5
trnR-ACG-trnN-GUU_p3	GGGAAACAGATCCAGTGGAG	TGGTCGTAGGTTCAAGTCCT	55.0	50.0	57.6	58.0	665-677 bp	1.0	80.3
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	57.8	57.7	668-680 bp	1.0	79.3
trnR-ACG-trnN-GUU_p5	TGGGAAACAGATCCAGTGGA	TTCCTCAGTAGCTCAGTGGT	50.0	50.0	58.3	57.7	709-721 bp	1.0	78.8