Hotspot

trnQ-UUG-accD

Genome · 56897-58109 · 1213 bp

Back to Meristotropis

Pi 0.0141

MarkerSeek score 56.10

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: Genome
length_bp: 1213
variable_sites: 17
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GAGGTTCGAATCCTTCCGTC	ACAGCATAGAATTGAACCACCA	55.0	40.9	58.1	58.0	1269-1273 bp	1.0	64.5
trnQ-UUG-accD_p2	TTCGGAGGTTCGAATCCTTC	ACAGCATAGAATTGAACCACCA	50.0	40.9	57.3	58.0	1273-1277 bp	1.0	59.8
trnQ-UUG-accD_p3	GAGGTTCGAATCCTTCCGTC	CAGCATAGAATTGAACCACCA	55.0	42.9	58.1	56.2	1268-1272 bp	1.0	58.6
trnQ-UUG-accD_p4	GAGGTTCGAATCCTTCCGTC	ACAGCATAGAATTGAACCACC	55.0	42.9	58.1	56.2	1269-1273 bp	1.0	58.5
trnQ-UUG-accD_p5	GGTTTTGGTCCCGTTATTCG	ACAGCATAGAATTGAACCACCA	50.0	40.9	57.1	58.0	1289-1293 bp	1.0	57.9