Hotspot

atpH-atpF

Genome · 49717-50219 · 503 bp

Back to Meristotropis

Pi 0.0142

MarkerSeek score 66.00

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: Genome
length_bp: 503
variable_sites: 7
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	AGAGGGAAAAATCCGGGGTA	GATGGCCAGTAACTCAAGCA	50.0	50.0	58.0	57.9	662-677 bp	1.0	80.9
atpH-atpF_p2	GAGGGAAAAATCCGGGGTAC	GATGGCCAGTAACTCAAGCA	55.0	50.0	58.0	57.9	661-676 bp	1.0	80.7
atpH-atpF_p3	AAGCAGAGGGAAAAATCCGG	GATGGCCAGTAACTCAAGCA	50.0	50.0	58.2	57.9	666-681 bp	1.0	79.8
atpH-atpF_p4	CCGGAAGCAGAGGGAAAAAT	GATGGCCAGTAACTCAAGCA	50.0	50.0	58.2	57.9	670-685 bp	1.0	79.8
atpH-atpF_p5	AGAGGGAAAAATCCGGGGTA	CGGATGGCCAGTAACTCAAG	50.0	55.0	58.0	58.3	664-679 bp	1.0	79.1