Hotspot

psbD-trnT-GGU

Genome · 29636-31086 · 1451 bp

Back to Meristotropis

Pi 0.00968

MarkerSeek score 61.20

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1451
variable_sites: 14
pi: 0.00968
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	GAGCAATAGACCGGACCAAC	CAGTGTGGTAGAGTAACGCC	55.0	55.0	58.3	58.0	1627-1677 bp	1.0	78.0
psbD-trnT-GGU_p2	GAGCAATAGACCGGACCAAC	GTGGTAGAGTAACGCCATGG	55.0	55.0	58.3	58.1	1623-1673 bp	1.0	77.6
psbD-trnT-GGU_p3	AAGAGCAATAGACCGGACCA	CAGTGTGGTAGAGTAACGCC	50.0	55.0	58.4	58.0	1629-1679 bp	1.0	77.4
psbD-trnT-GGU_p4	AGAGCAATAGACCGGACCAA	CAGTGTGGTAGAGTAACGCC	50.0	55.0	58.4	58.0	1628-1678 bp	1.0	77.4
psbD-trnT-GGU_p5	AGAGCAATAGACCGGACCAA	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.4	58.1	1624-1674 bp	1.0	76.9