Hotspot

trnV-UAC-ndhC

Genome · 10547-11001 · 455 bp

Back to Meristotropis

Pi 0.0176

MarkerSeek score 63.90

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: Genome
length_bp: 455
variable_sites: 8
pi: 0.0176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	AGAAGGTCTACGGTTCGAGT	TTCTGGCCCTTTGCTAATCG	50.0	50.0	57.8	58.2	605-653 bp	1.0	77.4
trnV-UAC-ndhC_p2	GAAGGTCTACGGTTCGAGTC	TTCTGGCCCTTTGCTAATCG	55.0	50.0	57.5	58.2	604-652 bp	1.0	74.7
trnV-UAC-ndhC_p3	GTTCGAGTCCGTATAGCCCT	TTCTGGCCCTTTGCTAATCG	55.0	50.0	58.7	58.2	593-641 bp	1.0	73.6
trnV-UAC-ndhC_p4	AAGGTCTACGGTTCGAGTCC	TTCTGGCCCTTTGCTAATCG	55.0	50.0	58.8	58.2	603-651 bp	1.0	72.4
trnV-UAC-ndhC_p5	GAGAAGGTCTACGGTTCGAG	TTCTGGCCCTTTGCTAATCG	55.0	50.0	57.2	58.2	606-654 bp	1.0	72.4