Hotspot

trnK-UUU-rbcL

Genome · 4358-5439 · 1082 bp

Back to Meristotropis

Pi 0.017

MarkerSeek score 70.50

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: Genome
length_bp: 1082
variable_sites: 18
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	TGGAGTCGCACTTAAAAGCC	CGTCAAGCTTGCAAATGGAG	50.0	50.0	58.5	58.0	1223-1227 bp	1.0	78.6
trnK-UUU-rbcL_p2	TGGAGTCGCACTTAAAAGCC	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.5	58.0	1205-1209 bp	1.0	78.6
trnK-UUU-rbcL_p3	AACCCTACCGATGGTATGGA	CGTCAAGCTTGCAAATGGAG	50.0	50.0	57.5	58.0	1276-1280 bp	1.0	78.5
trnK-UUU-rbcL_p4	AACCCTACCGATGGTATGGA	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.5	58.0	1258-1262 bp	1.0	78.5
trnK-UUU-rbcL_p5	TGGAGTCGCACTTAAAAGCC	TTTGAATTCCCGGCAATGGA	50.0	45.0	58.5	58.1	1160-1164 bp	1.0	78.4