Hotspot

ycf1

SSC · 128551-134256 · 5706 bp

Back to Mentzelia

Pi 0.0375

MarkerSeek score 60.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5706
variable_sites: 212
pi: 0.0375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATGCTCCACTGGTACCTCTT	ACGAAGGGGACTAAACAGGA	50.0	50.0	58.0	58.0	1173-1182 bp	1.0	84.0
ycf1_p2	ATGCTCCACTGGTACCTCTT	CGAAAAACCGCTTGTGACTC	50.0	50.0	58.0	58.0	898 bp	0.5	59.5
ycf1_p3	CCACTGGTACCTCTTCTGGA	CGAAAAACCGCTTGTGACTC	55.0	50.0	58.1	58.0	893 bp	0.5	59.4
ycf1_p4	TCCACTGGTACCTCTTCTGG	CGAAAAACCGCTTGTGACTC	55.0	50.0	58.1	58.0	894 bp	0.5	59.4
ycf1_p5	CCACTGGTACCTCTTCTGGA	ACGAAGGGGACTAAACAGGA	55.0	50.0	58.1	58.0	1177 bp	0.5	59.4