Hotspot

ndhF

SSC · 114343-116565 · 2223 bp

Back to Mentzelia

Pi 0.0328

MarkerSeek score 61.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 73
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	ATCAAGTACCGGTTTGGGTG	50.0	50.0	58.0	57.8	2855 bp	0.5	58.3
ndhF_p2	TGGTTCGAAAAACCGCTTCT	AATTGGCCAAGCTCAAGGAA	45.0	45.0	58.3	58.0	2936 bp	0.5	57.3
ndhF_p3	TGGTTCGAAAAACCGCTTCT	ATTGGCCAAGCTCAAGGAAA	45.0	45.0	58.3	58.0	2935 bp	0.5	57.3
ndhF_p4	AAGAGCTTCCGAAACGAAGG	ATCAAGTACCGGTTTGGGTG	50.0	50.0	58.2	57.8	2868 bp	0.5	56.8
ndhF_p5	TGGTTCGAAAAACCGCTTCT	GCCAAGCTCAAGGAAAGAGT	45.0	50.0	58.3	58.1	2931 bp	0.5	56.6