Hotspot

rps15-ycf1

SSC · 128150-128550 · 401 bp

Back to Mentzelia

Pi 0.0551

MarkerSeek score 70.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 401
variable_sites: 22
pi: 0.0551
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCGCAGACCTCTCTGAGATA	AACCCCTTCGTCTGTCTGTA	55.0	50.0	58.0	58.0	1075-1080 bp	1.0	83.3
rps15-ycf1_p2	CCGCAGACCTCTCTGAGATA	ACCCCTTCGTCTGTCTGTAA	55.0	50.0	58.0	58.0	1074-1079 bp	1.0	83.3
rps15-ycf1_p3	AAGTCGTTGACGTTTTCCCA	AACCCCTTCGTCTGTCTGTA	45.0	50.0	58.0	58.0	1102-1107 bp	1.0	83.2
rps15-ycf1_p4	AAGTCGTTGACGTTTTCCCA	ACCCCTTCGTCTGTCTGTAA	45.0	50.0	58.0	58.0	1101-1106 bp	1.0	83.2
rps15-ycf1_p5	CCGCAGACCTCTCTGAGATA	AATGGCAGTCGTTTCAGTGT	55.0	45.0	58.0	58.0	611 bp	0.5	59.5