Hotspot

ycf1-ndhF

IRb · 114181-114342 · 162 bp

Back to Mentzelia

Pi 0.112

MarkerSeek score 75.40

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: IRb
length_bp: 162
variable_sites: 16
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACGAAGGGGACTAAACAGGA	TCAGATATGTAGGGAGCGGG	50.0	55.0	58.0	58.1	381-400 bp	1.0	87.8
ycf1-ndhF_p2	ACGAAGGGGACTAAACAGGA	TTTGTGGGAGAAGGCATCAG	50.0	50.0	58.0	57.8	397-416 bp	1.0	86.5
ycf1-ndhF_p3	ACGAAGGGGACTAAACAGGA	CTTTGTGGGAGAAGGCATCA	50.0	50.0	58.0	57.8	398-417 bp	1.0	86.4
ycf1-ndhF_p4	AAGAGCTTCCGAAACGAAGG	TCAGATATGTAGGGAGCGGG	50.0	55.0	58.2	58.1	394-413 bp	1.0	86.3
ycf1-ndhF_p5	TGCGGAATCTCAGCATTCAA	TCAGATATGTAGGGAGCGGG	45.0	55.0	57.9	58.1	833-852 bp	1.0	82.6