Hotspot

petD-rpoA

LSC · 81725-81925 · 201 bp

Back to Mentzelia

Pi 0.05

MarkerSeek score 74.90

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 201
variable_sites: 9
pi: 0.05
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TGGGTATTGGCGCAACATTA	GAAAAGCAGTTCGCAAGTGA	45.0	45.0	57.9	57.2	278-299 bp	1.0	79.4
petD-rpoA_p2	TGGGTATTGGCGCAACATTA	AGAAAAGCAGTTCGCAAGTG	45.0	45.0	57.9	57.2	279-300 bp	1.0	79.3
petD-rpoA_p3	GGGTATTGGCGCAACATTAC	GAAAAGCAGTTCGCAAGTGA	50.0	45.0	57.5	57.2	277-298 bp	1.0	76.6
petD-rpoA_p4	GGTATTGGCGCAACATTACC	GAAAAGCAGTTCGCAAGTGA	50.0	45.0	57.5	57.2	276-297 bp	1.0	76.6
petD-rpoA_p5	GGTATTGGCGCAACATTACC	AGAAAAGCAGTTCGCAAGTG	50.0	45.0	57.5	57.2	277-298 bp	1.0	76.5