Hotspot

psaJ-rpl33

LSC · 71098-71539 · 442 bp

Back to Mentzelia

Pi 0.0468

MarkerSeek score 75.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 442
variable_sites: 20
pi: 0.0468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	GGACATCTTTACCCTTGGCC	45.0	55.0	58.1	58.2	488-496 bp	1.0	83.2
psaJ-rpl33_p2	TGTGGCACCGGTACTAAGTA	GGACATCTTTACCCTTGGCC	50.0	55.0	58.1	58.2	565-573 bp	1.0	82.4
psaJ-rpl33_p3	TGGCACCGGTACTAAGTACT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	563-571 bp	1.0	81.3
psaJ-rpl33_p4	GTTCGGGTCTTTAGCAGGTC	GGACATCTTTACCCTTGGCC	55.0	55.0	58.3	58.2	538-546 bp	1.0	81.1
psaJ-rpl33_p5	GTGGCACCGGTACTAAGTAC	GGACATCTTTACCCTTGGCC	55.0	55.0	57.7	58.2	564-572 bp	1.0	80.8