Hotspot

petA-psbJ

LSC · 66615-67641 · 1027 bp

Back to Mentzelia

Pi 0.0647

MarkerSeek score 78.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1027
variable_sites: 66
pi: 0.0647
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	56.8	57.6	1087-1108 bp	1.0	73.1
petA-psbJ_p2	CGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	47.6	50.0	58.3	57.6	1087-1108 bp	1.0	71.9
petA-psbJ_p3	TCGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	47.6	50.0	58.3	57.6	1088-1109 bp	1.0	71.9
petA-psbJ_p4	GGCATCTGTTATTTTGGCACA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.4	57.6	1138-1159 bp	1.0	69.4
petA-psbJ_p5	TGGCATCTGTTATTTTGGCAC	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.4	57.6	1139-1160 bp	1.0	69.4