Hotspot

trnF-GAA-ndhJ

LSC · 51000-51701 · 702 bp

Back to Mentzelia

Pi 0.0486

MarkerSeek score 80.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 34
pi: 0.0486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	844-878 bp	1.0	82.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	808-842 bp	1.0	82.4
trnF-GAA-ndhJ_p3	ATGATGCATCGGGAATGGTC	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	864-898 bp	1.0	81.8
trnF-GAA-ndhJ_p4	GATGATGCATCGGGAATGGT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	865-899 bp	1.0	81.8
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	832-866 bp	1.0	81.6