Hotspot

psaB-psaA

LSC · 41756-41780 · 25 bp

Back to Mentzelia

Pi 0.04
MarkerSeek score 73.70
Primer available yes
Label psaB-psaA

Feature Metadata

label_name
psaB-psaA
feature_type
igs
region
LSC
length_bp
25
variable_sites
1
pi
0.04
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaB-psaA_p1 AGCTAAGCCTTGGCTAAACC GTACAAGGACGTGCTGTAGG 50.0 55.0 57.9 58.0 160 bp 1.0 79.4
psaB-psaA_p2 GCTAAGCCTTGGCTAAACCT GTACAAGGACGTGCTGTAGG 50.0 55.0 57.9 58.0 159 bp 1.0 79.4
psaB-psaA_p3 AATACGACGAGTAGTGGGGT GTACAAGGACGTGCTGTAGG 50.0 55.0 57.9 58.0 184 bp 1.0 79.3
psaB-psaA_p4 GTAGTGGGGTCCTGAGCTAA GTACAAGGACGTGCTGTAGG 55.0 55.0 58.1 58.0 174 bp 1.0 79.3
psaB-psaA_p5 TAGTGGGGTCCTGAGCTAAG GTACAAGGACGTGCTGTAGG 55.0 55.0 57.8 58.0 173 bp 1.0 79.1