Hotspot

trnC-GCA-petN

LSC · 29305-30153 · 849 bp

Back to Mentzelia

Pi 0.0535

MarkerSeek score 82.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 44
pi: 0.0535
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACGAGCGAAAGGGAAAATGT	50.0	45.0	58.1	58.1	935-953 bp	1.0	84.5
trnC-GCA-petN_p2	TTTTGTATCGTTTTGGCGGC	ACGAGCGAAAGGGAAAATGT	45.0	45.0	57.9	58.1	991-1009 bp	1.0	84.4
trnC-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	CCATACTACGAGCGAAAGGG	45.0	55.0	57.9	57.9	998-1016 bp	1.0	84.1
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	CCCATACTACGAGCGAAAGG	45.0	55.0	57.9	57.9	999-1017 bp	1.0	84.1
trnC-GCA-petN_p5	TTTTGTATCGTTTTGGCGGC	CCCCATACTACGAGCGAAAG	45.0	55.0	57.9	57.9	1000-1018 bp	1.0	84.1