Hotspot

atpH-atpI

LSC · 14589-15746 · 1158 bp

Back to Mentzelia

Pi 0.0352

MarkerSeek score 75.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1158
variable_sites: 39
pi: 0.0352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CAAGCGGTATCCAAGCTCTT	45.0	50.0	58.0	58.0	1254-1267 bp	1.0	85.1
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CAAGCGGTATCCAAGCTCTT	50.0	50.0	57.9	58.0	1310-1323 bp	1.0	84.3
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	CAAGCGGTATCCAAGCTCTT	45.0	50.0	58.2	58.0	1255-1268 bp	1.0	83.9
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	CAAGCGGTATCCAAGCTCTT	55.0	50.0	58.2	58.0	1314-1327 bp	1.0	83.6
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	CAAGCGGTATCCAAGCTCTT	50.0	50.0	57.8	58.0	1269-1282 bp	1.0	83.6