Hotspot

trnR-UCU-atpA

LSC · 10960-11105 · 146 bp

Back to Mentzelia

Pi 0.0703

MarkerSeek score 77.40

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 146
variable_sites: 9
pi: 0.0703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATCGGAATGAAAAGCGTCCA	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	57.9	58.1	288-306 bp	1.0	84.9
trnR-UCU-atpA_p2	TCGGAATGAAAAGCGTCCAT	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	57.9	58.1	287-305 bp	1.0	84.9
trnR-UCU-atpA_p3	ATCGGAATGAAAAGCGTCCA	CATTCACCGAGGAAGCAGAA	45.0	50.0	57.9	57.9	296-314 bp	1.0	84.7
trnR-UCU-atpA_p4	TCGGAATGAAAAGCGTCCAT	CATTCACCGAGGAAGCAGAA	45.0	50.0	57.9	57.9	295-313 bp	1.0	84.7
trnR-UCU-atpA_p5	TCGGAATGAAAAGCGTCCAT	AAGACATTCACCGAGGAAGC	45.0	50.0	57.9	57.9	299-317 bp	1.0	84.7