Hotspot

trnS-GCU-trnG-UCC

LSC · 9234-10004 · 771 bp

Back to Mentzelia

Pi 0.0374

MarkerSeek score 77.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 27
pi: 0.0374
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AATCCAAACCGAAAGACCCC	50.0	50.0	58.0	58.1	869-884 bp	1.0	85.8
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	AATCCAAACCGAAAGACCCC	50.0	50.0	58.0	58.1	868-883 bp	1.0	85.8
trnS-GCU-trnG-UCC_p3	CCATCAACGGAAAGAGAGGG	AATCCAAACCGAAAGACCCC	55.0	50.0	58.0	58.1	929-944 bp	1.0	85.4
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	AATCCAAACCGAAAGACCCC	50.0	50.0	58.2	58.1	923-938 bp	1.0	84.5
trnS-GCU-trnG-UCC_p5	AGCAATCCGACGCTTTAGTC	AATCCAAACCGAAAGACCCC	50.0	50.0	58.4	58.1	871-886 bp	1.0	82.9