Hotspot

ccsA-ndhD

SSC · 113618-113849 · 232 bp

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Pi 0.0215
MarkerSeek score 45.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
232
variable_sites
18
pi
0.0215
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TTGGAGTTGGGACCCAAAAG GGTTGATAATAGTGGGCGCA 50.0 50.0 57.9 58.0 1113-1119 bp 1.0 80.8
ccsA-ndhD_p2 TTGGAGTTGGGACCCAAAAG TGGTTGATAATAGTGGGCGC 50.0 50.0 57.9 58.0 1114-1120 bp 1.0 80.8
ccsA-ndhD_p3 CTAACGAAGCATGGGGATCC GGTTGATAATAGTGGGCGCA 55.0 50.0 58.1 58.0 1135-1141 bp 1.0 80.6
ccsA-ndhD_p4 CTAACGAAGCATGGGGATCC TGGTTGATAATAGTGGGCGC 55.0 50.0 58.1 58.0 1136-1142 bp 1.0 80.6
ccsA-ndhD_p5 ATTAGTACCCGCCCTTCAGT GGTTGATAATAGTGGGCGCA 50.0 50.0 58.1 58.0 1500-1506 bp 1.0 80.1