Hotspot

rpl32-trnL-UAG

SSC · 111716-112458 · 743 bp

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Pi 0.0108
MarkerSeek score 55.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
743
variable_sites
27
pi
0.0108
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCTCTTTCTACCGGGACTTCA GATGATATGCCATGCCGCTA 47.6 50.0 57.8 58.0 896-914 bp 1.0 73.6
rpl32-trnL-UAG_p2 TCTCTTTCTACCGGGACTTCA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.8 57.9 843-861 bp 1.0 73.0
rpl32-trnL-UAG_p3 TCTCTTTCTACCGGGACTTCA AGCAGCGTGTCTACCAATTT 47.6 45.0 57.8 57.8 836-854 bp 1.0 72.5
rpl32-trnL-UAG_p4 TCTCTTTCTACCGGGACTTCA CCTAAGAGCAGCGTGTCTAC 47.6 55.0 57.8 57.8 842-860 bp 1.0 72.4
rpl32-trnL-UAG_p5 TCTCTTTCTACCGGGACTTCA CTAAGAGCAGCGTGTCTACC 47.6 55.0 57.8 57.8 841-859 bp 1.0 72.4