Hotspot

trnG-GCC-trnM-CAU

LSC · 35940-36103 · 164 bp

Back to Mentha

Pi 0.0136
MarkerSeek score 53.40
Primer available yes
Label trnG-GCC-trnM-CAU

Feature Metadata

label_name
trnG-GCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
164
variable_sites
9
pi
0.0136
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 225-230 bp 1.0 84.2
trnG-GCC-trnM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 226-231 bp 1.0 84.1
trnG-GCC-trnM-CAU_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 261-266 bp 1.0 83.6
trnG-GCC-trnM-CAU_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 262-267 bp 1.0 83.6
trnG-GCC-trnM-CAU_p5 ATGCGGATATGGTCGAATGG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.9 58.0 302-307 bp 1.0 82.7