Hotspot

rpoB-trnC-GCA

LSC · 26863-27983 · 1121 bp

Back to Mentha

Pi 0.00379
MarkerSeek score 56.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1121
variable_sites
16
pi
0.00379
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AGTTCTTCTGCCAAACCCTG TTTTGGTGATTCAGGCGACA 50.0 45.0 58.0 58.0 1151-1357 bp 1.0 84.7
rpoB-trnC-GCA_p2 GTTCTTCTGCCAAACCCTGA TTTTGGTGATTCAGGCGACA 50.0 45.0 58.0 58.0 1150-1356 bp 1.0 84.6
rpoB-trnC-GCA_p3 AGTTCTTCTGCCAAACCCTG CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1130-1336 bp 1.0 84.2
rpoB-trnC-GCA_p4 GTTCTTCTGCCAAACCCTGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1129-1335 bp 1.0 84.2
rpoB-trnC-GCA_p5 GCCAAACCCTGATCGATGAA TTTTGGTGATTCAGGCGACA 50.0 45.0 58.2 58.0 1142-1348 bp 1.0 82.8