Hotspot

rps2-rpoC2

LSC · 16230-16451 · 222 bp

Pi 0.0131

MarkerSeek score 57.10

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 222
variable_sites: 7
pi: 0.0131
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCTCCAGCATCTCTTCCAAG	TTCGAGGGGGAAATGAGAGA	55.0	50.0	57.7	57.8	380-385 bp	1.0	78.1
rps2-rpoC2_p2	CCTCCAGCATCTCTTCCAAG	TCGAGGGGGAAATGAGAGAT	55.0	50.0	57.7	57.5	379-384 bp	1.0	76.3
rps2-rpoC2_p3	CATGGCCAAAATGAACTCCC	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.3	57.8	401-406 bp	1.0	75.2
rps2-rpoC2_p4	CCATGGCCAAAATGAACTCC	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.3	57.8	402-407 bp	1.0	75.2
rps2-rpoC2_p5	ATGGCCAAAATGAACTCCCG	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.8	57.8	400-405 bp	1.0	74.1