Hotspot

ycf1

SSC · 123311-128647 · 5337 bp

Back to Menonvillea

Pi 0.0202

MarkerSeek score 51.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5337
variable_sites: 108
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGCCCATGGAATCTGAGGTA	GTTTGGGGACTGGAAACTGA	50.0	50.0	57.8	57.6	1065-1071 bp	1.0	78.0
ycf1_p2	AGCCCATGGAATCTGAGGTA	TTGGGGACTGGAAACTGAAC	50.0	50.0	57.8	57.6	1063-1069 bp	1.0	78.0
ycf1_p3	TAGCCCATGGAATCTGAGGT	TTGGGGACTGGAAACTGAAC	50.0	50.0	57.8	57.6	1064-1070 bp	1.0	78.0
ycf1_p4	GCCGAATTTCCCCTATACCC	GTTTGGGGACTGGAAACTGA	55.0	50.0	57.8	57.6	1378-1384 bp	1.0	77.9
ycf1_p5	GCCGAATTTCCCCTATACCC	TTGGGGACTGGAAACTGAAC	55.0	50.0	57.8	57.6	1376-1382 bp	1.0	77.9