Hotspot

psbA

LSC · 329-1390 · 1062 bp

Back to Menonvillea

Pi 0.00847

MarkerSeek score 50.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 9
pi: 0.00847
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTAGGTTTAGACTAACGCT	GATTAAATAAACCAAGGATTTTACC	40.0	28.0	53.1	53.0	1113 bp	1.0	40.6
psbA_p2	AACTAGGTTTAGACTAACGCT	GATTAAATAAACCAAGGATTTTACC	38.1	28.0	54.1	53.0	1114 bp	1.0	40.6
psbA_p3	AAACTAGGTTTAGACTAACGCT	GATTAAATAAACCAAGGATTTTACC	36.4	28.0	54.9	53.0	1115 bp	1.0	40.6
psbA_p4	AGGTTTAGACTAACGCTAAAGA	GATTAAATAAACCAAGGATTTTACC	36.4	28.0	54.6	53.0	1110 bp	1.0	40.6
psbA_p5	AACTAGGTTTAGACTAACGC	GATTAAATAAACCAAGGATTTTACC	40.0	28.0	52.4	53.0	1114 bp	1.0	40.6