Hotspot

ndhI

SSC · 118726-119241 · 516 bp

Back to Menonvillea

Pi 0.0564

MarkerSeek score 72.70

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 516
variable_sites: 29
pi: 0.0564
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	GTGCAGCAGCTACAAAATGG	CGAAGACCTCATGTACGGTT	50.0	50.0	58.0	57.4	1648-1708 bp	1.0	78.5
ndhI_p2	GTGCAGCAGCTACAAAATGG	TTGTGGCGTCAACCTATAGG	50.0	50.0	58.0	57.3	1517-1577 bp	1.0	78.4
ndhI_p3	GCCCCCACGTAAATAAGGAG	CGAAGACCTCATGTACGGTT	55.0	50.0	58.0	57.4	1670-1730 bp	1.0	78.3
ndhI_p4	GTGCAGCAGCTACAAAATGG	CAGAATGCGAGAGGTTACCA	50.0	50.0	58.0	57.3	1468 bp	0.5	54.5
ndhI_p5	GCCCCCACGTAAATAAGGAG	CAGAATGCGAGAGGTTACCA	55.0	50.0	58.0	57.3	1490 bp	0.5	54.3