Hotspot

trnL-ycf5

SSC · 113814-113935 · 122 bp

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Pi 0.0984

MarkerSeek score 73.30

Primer available yes

Label trnL-ycf5

Feature Metadata

label_name: trnL-ycf5
feature_type: igs
region: SSC
length_bp: 122
variable_sites: 12
pi: 0.0984
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-ycf5_p1	GTTTTGAACAAGTTGCCGCT	TAACCACTGGGACTGGAGAG	45.0	55.0	58.1	58.1	633 bp	1.0	81.2
trnL-ycf5_p2	AAGTTGCCGCTATGGTGAAA	TAACCACTGGGACTGGAGAG	45.0	55.0	58.1	58.1	624 bp	1.0	81.1
trnL-ycf5_p3	TAGACACGCTGCTCTTAGGA	TAACCACTGGGACTGGAGAG	50.0	55.0	57.9	58.1	600 bp	1.0	80.9
trnL-ycf5_p4	AGTTGCCGCTATGGTGAAAT	TAACCACTGGGACTGGAGAG	45.0	55.0	57.9	58.1	623 bp	1.0	80.8
trnL-ycf5_p5	AAATTGGTAGACACGCTGCT	TAACCACTGGGACTGGAGAG	45.0	55.0	57.8	58.1	607 bp	1.0	80.4