Hotspot

psbT-psbN

LSC · 73320-73384 · 65 bp

Back to Menonvillea

Pi 0.0462

MarkerSeek score 72.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 3
pi: 0.0462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CCACGATCAAATTTATGGAAGCA	TTCGAGGAACACGGAGACTA	39.1	50.0	57.8	57.8	202 bp	1.0	53.7
psbT-psbN_p2	ACCACGATCAAATTTATGGAAGC	TTCGAGGAACACGGAGACTA	39.1	50.0	57.8	57.8	203 bp	1.0	53.7
psbT-psbN_p3	CCACGATCAAATTTATGGAAGCA	TCGAGGAACACGGAGACTAG	39.1	55.0	57.8	58.3	201 bp	1.0	53.1
psbT-psbN_p4	ACCACGATCAAATTTATGGAAGC	TCGAGGAACACGGAGACTAG	39.1	55.0	57.8	58.3	202 bp	1.0	53.1
psbT-psbN_p5	CCACGATCAAATTTATGGAAGCA	CATTCGAGGAACACGGAGAC	39.1	55.0	57.8	58.4	204 bp	1.0	52.2