Hotspot

petA-psbJ

LSC · 62094-63035 · 942 bp

Back to Menonvillea

Pi 0.0244

MarkerSeek score 63.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 942
variable_sites: 23
pi: 0.0244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGAAACAGTTTGAGAAGGTTCA	GCTGGTATTCCTGTGATCGG	36.4	55.0	55.8	58.1	1061-1062 bp	1.0	47.1
petA-psbJ_p2	AAGAAACAGTTTGAGAAGGTTCA	GCTGGTATTCCTGTGATCGG	34.8	55.0	56.5	58.1	1062-1063 bp	1.0	44.8
petA-psbJ_p3	AGAAACAGTTTGAGAAGGTTCAA	GCTGGTATTCCTGTGATCGG	34.8	55.0	56.5	58.1	1061-1062 bp	1.0	44.8
petA-psbJ_p4	TGAGAAGGTTCAATTATCCGA	GCTGGTATTCCTGTGATCGG	38.1	55.0	54.4	58.1	1051-1052 bp	1.0	43.7
petA-psbJ_p5	GAAACAGTTTGAGAAGGTTCA	GCTGGTATTCCTGTGATCGG	38.1	55.0	54.3	58.1	1060-1061 bp	1.0	42.9