Hotspot

trnD-trnY

LSC · 29629-30063 · 435 bp

Back to Menonvillea

Pi 0.0437

MarkerSeek score 72.90

Primer available yes

Label trnD-trnY

Feature Metadata

label_name: trnD-trnY
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 19
pi: 0.0437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-trnY_p1	CCAGAAACACAAAGGGGAGT	GCAAGATGTCTACGCTGGTT	50.0	50.0	57.6	58.3	593-596 bp	1.0	77.5
trnD-trnY_p2	CCAGAAACACAAAGGGGAGT	GTTGGCAAGATGTCTACGCT	50.0	50.0	57.6	58.3	597-600 bp	1.0	77.4
trnD-trnY_p3	CCAGAAACACAAAGGGGAGT	TTGGCAAGATGTCTACGCTG	50.0	50.0	57.6	58.3	596-599 bp	1.0	77.4
trnD-trnY_p4	CCAGAAACACAAAGGGGAGT	GGGGACGGACTGTAAATTCG	50.0	55.0	57.6	58.4	616-619 bp	1.0	76.7
trnD-trnY_p5	CCAGAAACACAAAGGGGAGT	GGGACGGACTGTAAATTCGT	50.0	50.0	57.6	57.6	615-618 bp	1.0	76.5