Hotspot

atpF-atpH

LSC · 12659-13132 · 474 bp

Back to Menonvillea

Pi 0.0496

MarkerSeek score 86.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 474
variable_sites: 23
pi: 0.0496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	582-592 bp	1.0	80.2
atpF-atpH_p2	CCAGTGACCCAAGTAAACGA	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.5	57.6	555-565 bp	1.0	77.1
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TTTATGGCCTGGTTGTAGCA	50.0	45.0	57.9	57.1	586-596 bp	1.0	76.4
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCCTGGTTGTAGCATTAGC	50.0	55.0	57.9	59.0	581-591 bp	1.0	75.8
atpF-atpH_p5	GGCCAGTGACCCAAGTAAAC	TGGCCTGGTTGTAGCATTAG	55.0	50.0	58.8	57.6	557-567 bp	1.0	75.3