Hotspot

ycf1

SSC · 123990-129317 · 5328 bp

Pi 0.0296

MarkerSeek score 59.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5328
variable_sites: 232
pi: 0.0296
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAACGAATTCCAGGCTCCAT	AGGGGAGGATCTGGACAAAA	50.0	50.0	58.0	57.9	1056-1059 bp	1.0	83.9
ycf1_p2	GTTTTACGCCTTTGGGAACG	AAGGGGAGGATCTGGACAAA	50.0	50.0	58.0	57.9	1240-1243 bp	1.0	83.6
ycf1_p3	GTTTTACGCCTTTGGGAACG	AGGGGAGGATCTGGACAAAA	50.0	50.0	58.0	57.9	1239-1242 bp	1.0	83.6
ycf1_p4	GTTTTACGCCTTTGGGAACG	TTTTGTATGGGATCTCGCGG	50.0	50.0	58.0	58.1	622 bp	0.333333	52.6
ycf1_p5	GAACGAATTCCAGGCTCCAT	TTTTGTATGGGATCTCGCGG	50.0	50.0	58.0	58.1	439 bp	0.333333	52.6