Hotspot

rpl16

LSC · 80944-82453 · 1510 bp

Pi 0.0267

MarkerSeek score 61.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1510
variable_sites: 59
pi: 0.0267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	GTATGGAGGGAATTGCACGT	50.0	50.0	58.0	58.0	2361-2379 bp	1.0	82.5
rpl16_p2	ACACATCAATTCTCGAGCCC	TGCACGTGTTGAATGGATCA	50.0	45.0	58.0	58.1	2009-2027 bp	1.0	82.2
rpl16_p3	GTCTCAATTCCCGTGGGATC	GTATGGAGGGAATTGCACGT	55.0	50.0	58.0	58.0	2620-2638 bp	1.0	82.1
rpl16_p4	ATCGGCGATTACTAGCTCCT	GTATGGAGGGAATTGCACGT	50.0	50.0	58.1	58.0	2392-2410 bp	1.0	82.0
rpl16_p5	GTCTCAATTCCCGTGGGATC	TGCACGTGTTGAATGGATCA	55.0	45.0	58.0	58.1	2268-2286 bp	1.0	81.8