Hotspot

rpl32-trnL

SSC · 113649-114438 · 790 bp

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Pi 0.079
MarkerSeek score 84.00
Primer available yes
Label rpl32-trnL

Feature Metadata

label_name
rpl32-trnL
feature_type
igs
region
SSC
length_bp
790
variable_sites
91
pi
0.079
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL_p1 TCTCGTACGGATTTCAAACGT TCCTAAGAGCAGCGTGTCTA 42.9 50.0 57.7 57.9 1211-1228 bp 1.0 77.8
rpl32-trnL_p2 TCTCGTACGGATTTCAAACGT AGCAGCGTGTCTACCAATTT 42.9 45.0 57.7 57.8 1204-1221 bp 1.0 77.3
rpl32-trnL_p3 TCTCGTACGGATTTCAAACGT CTAAGAGCAGCGTGTCTACC 42.9 55.0 57.7 57.8 1209-1226 bp 1.0 77.2
rpl32-trnL_p4 TCTCGTACGGATTTCAAACGT TAACCACTGGGACTGGAGAG 42.9 55.0 57.7 58.1 1785-1801 bp 1.0 75.8
rpl32-trnL_p5 TCTCGTACGGATTTCAAACGT TGCAGACTGAGGCATTTTGT 42.9 45.0 57.7 58.0 1752-1759 bp 0.666667 60.7