Hotspot

rps19-rpl2

IRb · 84086-84140 · 55 bp

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Pi 0.0494
MarkerSeek score 73.20
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
IRb
length_bp
55
variable_sites
4
pi
0.0494
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 TCCGCCTTTGTATTAAGCTTCT TTGATTCTTCGTCGCCGTAG 40.9 50.0 57.8 58.1 161-162 bp 1.0 64.0
rps19-rpl2_p2 TCCGCCTTTGTATTAAGCTTCT TTTGATTCTTCGTCGCCGTA 40.9 45.0 57.8 57.6 162-163 bp 1.0 61.9
rps19-rpl2_p3 CCGCCTTTGTATTAAGCTTCTC TTGATTCTTCGTCGCCGTAG 45.5 50.0 57.5 58.1 160-161 bp 1.0 61.4
rps19-rpl2_p4 TCCGCCTTTGTATTAAGCTTC TTGATTCTTCGTCGCCGTAG 42.9 50.0 56.3 58.1 161-162 bp 1.0 60.3
rps19-rpl2_p5 TGATTTCTTTTTCCGCCTTTGT TTGATTCTTCGTCGCCGTAG 36.4 50.0 57.3 58.1 172-173 bp 1.0 60.2