Hotspot

psbE-ORF31

LSC · 64143-65488 · 1346 bp

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Pi 0.0374
MarkerSeek score 73.00
Primer available yes
Label psbE-ORF31

Feature Metadata

label_name
psbE-ORF31
feature_type
igs
region
LSC
length_bp
1346
variable_sites
74
pi
0.0374
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-ORF31_p1 ATAACCAGCCCGCAATGAAT AGCTGCTAGTAGAAAACCGA 45.0 45.0 57.9 56.3 1497-1562 bp 1.0 70.9
psbE-ORF31_p2 AGAACGTTCTCCTGTGCTTC AGCTGCTAGTAGAAAACCGA 50.0 45.0 57.8 56.3 1415-1480 bp 1.0 70.6
psbE-ORF31_p3 AATAACCAGCCCGCAATGAA AGCTGCTAGTAGAAAACCGA 45.0 45.0 58.2 56.3 1498-1563 bp 1.0 70.3
psbE-ORF31_p4 GTTCTCCTGTGCTTCCAGAC AGCTGCTAGTAGAAAACCGA 55.0 45.0 58.2 56.3 1410-1475 bp 1.0 70.2
psbE-ORF31_p5 ATAACCAGCCCGCAATGAAT AGCTGCTAGTAGAAAACCGAA 45.0 42.9 57.9 57.0 1497-1562 bp 1.0 69.0