Hotspot

petA-psbJ

LSC · 62412-63356 · 945 bp

Back to Menkea

Pi 0.0406
MarkerSeek score 78.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
945
variable_sites
55
pi
0.0406
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TAGGATCGGTTGTTTTGGCA CAGGATTAGGTTCATCCCTGT 45.0 47.6 57.4 56.8 1054-1128 bp 1.0 64.7
petA-psbJ_p2 TAGGATCGGTTGTTTTGGCA TCAGGATTAGGTTCATCCCTGT 45.0 45.5 57.4 58.3 1055-1129 bp 1.0 64.4
petA-psbJ_p3 TAGGATCGGTTGTTTTGGCA TCAGGATTAGGTTCATCCCTG 45.0 47.6 57.4 56.5 1055-1129 bp 1.0 62.4
petA-psbJ_p4 AGGATCGGTTGTTTTGGCAC CAGGATTAGGTTCATCCCTGT 50.0 47.6 59.0 56.8 1053-1127 bp 1.0 60.9
petA-psbJ_p5 AGGATCGGTTGTTTTGGCAC TCAGGATTAGGTTCATCCCTGT 50.0 45.5 59.0 58.3 1054-1128 bp 1.0 60.6