Hotspot

trnF-ndhJ

LSC · 47976-48558 · 583 bp

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Pi 0.0427
MarkerSeek score 74.10
Primer available yes
Label trnF-ndhJ

Feature Metadata

label_name
trnF-ndhJ
feature_type
igs
region
LSC
length_bp
583
variable_sites
37
pi
0.0427
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-ndhJ_p1 CCTCGTGTCACCAGTTCAAA CCCCCAATTTTTATGAAATACAAGA 50.0 32.0 58.1 56.1 652-656 bp 0.666667 30.0
trnF-ndhJ_p2 CCTCGTGTCACCAGTTCAAA CCCCAATTTTTATGAAATACAAGATGC 50.0 33.3 58.1 58.2 651-655 bp 0.666667 30.0
trnF-ndhJ_p3 CCTCGTGTCACCAGTTCAAA CCCCCAATTTTTATGAAATACAAG 50.0 33.3 58.1 54.7 652-656 bp 0.666667 30.0
trnF-ndhJ_p4 CCTCGTGTCACCAGTTCAAA CCCCCAATTTTTATGAAATACAAGATG 50.0 33.3 58.1 57.7 652-656 bp 0.666667 30.0
trnF-ndhJ_p5 AATCCTCGTGTCACCAGTTC CCCCCAATTTTTATGAAATACAAGA 50.0 32.0 57.5 56.1 655-659 bp 0.666667 30.0