Hotspot

trnT-trnL

LSC · 45773-46558 · 786 bp

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Pi 0.0388
MarkerSeek score 77.00
Primer available yes
Label trnT-trnL

Feature Metadata

label_name
trnT-trnL
feature_type
igs
region
LSC
length_bp
786
variable_sites
44
pi
0.0388
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-trnL_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCAATTCC 55.0 55.0 57.9 58.2 831-848 bp 1.0 84.7
trnT-trnL_p2 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 834-851 bp 1.0 84.0
trnT-trnL_p3 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 863-880 bp 1.0 83.6
trnT-trnL_p4 GGAATCGAACCGATGACCAT AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 866-883 bp 1.0 82.9
trnT-trnL_p5 GCGATGCTCTAACCTCTGAG TAAGTCCGTAGCGTCTACCA 55.0 50.0 57.9 57.6 836-853 bp 1.0 82.7