Hotspot

trnS-rps4

LSC · 44388-44703 · 316 bp

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Pi 0.0316
MarkerSeek score 70.20
Primer available yes
Label trnS-rps4

Feature Metadata

label_name
trnS-rps4
feature_type
igs
region
LSC
length_bp
316
variable_sites
15
pi
0.0316
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-rps4_p1 GAGTGGTTGAAGGCGTAACA AGGAAATGCGTCGGTTTGAA 50.0 45.0 58.1 58.4 457 bp 1.0 78.4
trnS-rps4_p2 GAGTGGTTGAAGGCGTAACA GGAAATGCGTCGGTTTGAAA 50.0 45.0 58.1 57.6 456 bp 1.0 78.3
trnS-rps4_p3 GAGTGGTTGAAGGCGTAACA TAGGAAATGCGTCGGTTTGA 50.0 45.0 58.1 57.5 458 bp 1.0 77.9
trnS-rps4_p4 TCCTGATGTCGGAGAGATGG AGGAAATGCGTCGGTTTGAA 55.0 45.0 58.3 58.4 479 bp 1.0 76.9
trnS-rps4_p5 TCCTGATGTCGGAGAGATGG GGAAATGCGTCGGTTTGAAA 55.0 45.0 58.3 57.6 478 bp 1.0 76.8