Hotspot

psbI-trnS

LSC · 7628-7716 · 89 bp

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Pi 0.0449
MarkerSeek score 74.50
Primer available yes
Label psbI-trnS

Feature Metadata

label_name
psbI-trnS
feature_type
igs
region
LSC
length_bp
89
variable_sites
6
pi
0.0449
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS_p1 TCCTATCTAATGATCCAGGACGT TAGGAGAGATGGCTGAGTGG 43.5 55.0 58.0 57.9 224-231 bp 1.0 57.8
psbI-trnS_p2 TCCTATCTAATGATCCAGGACGT TGAGTGGACTAAAGCGTTGG 43.5 50.0 58.0 57.8 211-218 bp 1.0 57.3
psbI-trnS_p3 TCCTATCTAATGATCCAGGACGT GCTGAGTGGACTAAAGCGTT 43.5 50.0 58.0 58.2 213-220 bp 1.0 56.9
psbI-trnS_p4 TCCTATCTAATGATCCAGGACGT CGAGTTAATCGTACCGAGGG 43.5 55.0 58.0 57.6 174-181 bp 1.0 56.0
psbI-trnS_p5 TCCTATCTAATGATCCAGGACGT ATGGCTGAGTGGACTAAAGC 43.5 50.0 58.0 57.6 216-223 bp 1.0 55.3