Hotspot

trnK-rps16

LSC · 4309-4984 · 676 bp

Back to Menkea

Pi 0.0421
MarkerSeek score 80.20
Primer available yes
Label trnK-rps16

Feature Metadata

label_name
trnK-rps16
feature_type
igs
region
LSC
length_bp
676
variable_sites
40
pi
0.0421
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-rps16_p1 AAAGCCGAGTACTCTACCGT CGCTCAACCAACAAGAACAG 50.0 50.0 58.2 57.6 731-765 bp 1.0 83.8
trnK-rps16_p2 AAGCCGAGTACTCTACCGTT CGCTCAACCAACAAGAACAG 50.0 50.0 58.2 57.6 730-764 bp 1.0 83.8
trnK-rps16_p3 TACCGTTGAGTTAGCAACCC CGCTCAACCAACAAGAACAG 50.0 50.0 57.5 57.6 717-751 bp 1.0 81.5
trnK-rps16_p4 AGTCGCACTTAAAAGCCGAG CGCTCAACCAACAAGAACAG 50.0 50.0 58.6 57.6 742-776 bp 1.0 80.5
trnK-rps16_p5 TCGCACTTAAAAGCCGAGTA CGCTCAACCAACAAGAACAG 45.0 50.0 57.3 57.6 740-774 bp 1.0 79.3