Hotspot

rrn23

SSC · 108747-111550 · 2804 bp

Back to Menispermum

Pi 0.0132

MarkerSeek score 63.60

Primer available yes

Label rrn23

Feature Metadata

label_name: rrn23
feature_type: rRNA
region: SSC
length_bp: 2804
variable_sites: 37
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23_p1	TCCTTCCATTTAGCAGCACC	AAACTTCTGTCGCTCCATCC	50.0	50.0	57.9	57.9	2943 bp	1.0	79.2
rrn23_p2	CCTTCCATTTAGCAGCACCT	AAACTTCTGTCGCTCCATCC	50.0	50.0	57.9	57.9	2942 bp	1.0	79.1
rrn23_p3	CGATTCGGCAGTTCGATCTA	AAACTTCTGTCGCTCCATCC	50.0	50.0	57.6	57.9	2992 bp	1.0	77.0
rrn23_p4	AGCCTTAAAGTTAACGGCGA	AAACTTCTGTCGCTCCATCC	45.0	50.0	57.5	57.9	2911 bp	1.0	76.5
rrn23_p5	GCACCTTAGGATGGCATAGC	AAACTTCTGTCGCTCCATCC	55.0	50.0	58.5	57.9	2928 bp	1.0	76.4