Hotspot

rpl32-trnL-UAG

SSC · 117383-118257 · 875 bp

Back to Menispermum

Pi 0.0344

MarkerSeek score 64.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 875
variable_sites: 30
pi: 0.0344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CAAAGCGTTTTGGGCAGTAC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	1023-1049 bp	1.0	79.9
rpl32-trnL-UAG_p2	CAAAGCGTTTTGGGCAGTAC	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.2	57.8	1016-1042 bp	1.0	79.4
rpl32-trnL-UAG_p3	CAAAGCGTTTTGGGCAGTAC	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.2	57.8	1022-1048 bp	1.0	79.3
rpl32-trnL-UAG_p4	CAAAGCGTTTTGGGCAGTAC	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.2	57.8	1021-1047 bp	1.0	79.3
rpl32-trnL-UAG_p5	AAAGCGTTTTGGGCAGTACT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.3	57.9	1022-1048 bp	1.0	79.3