Hotspot

ycf4-cemA

LSC · 64898-65619 · 722 bp

Back to Menispermum

Pi 0.0295

MarkerSeek score 77.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 21
pi: 0.0295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.9	58.1	818-828 bp	1.0	81.7
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.1	58.1	820-830 bp	1.0	81.5
ycf4-cemA_p3	AAAAAGCTGCTGAATTGGCC	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	845-855 bp	1.0	80.9
ycf4-cemA_p4	AAAGCTGCTGAATTGGCCTA	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	843-853 bp	1.0	80.5
ycf4-cemA_p5	TGCGCGTACCAATTGAAGTA	GAGAGAGAGATCCACCAGGG	45.0	60.0	57.9	58.3	824-834 bp	1.0	80.0