Hotspot

accD-psaI

LSC · 63059-63804 · 746 bp

Back to Menispermum

Pi 0.0256

MarkerSeek score 71.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 19
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTTATTTCAGCTGCATGGGT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.9	58.0	860-885 bp	1.0	73.1
accD-psaI_p2	AGTTATTTCAGCTGCATGGGT	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.9	57.9	866-891 bp	1.0	72.4
accD-psaI_p3	TTTCAGCTGCATGGGTTTTT	CCATTGCAATTGCCGGAAAT	40.0	45.0	56.7	58.0	855-880 bp	1.0	71.4
accD-psaI_p4	TTTCAGCTGCATGGGTTTTT	AAGAAGCCATTGCAATTGCC	40.0	45.0	56.7	57.9	861-886 bp	1.0	70.8
accD-psaI_p5	AGTTATTTCAGCTGCATGGGT	AGGCACCAAAATAGAGGGGA	42.9	50.0	57.9	58.3	826-851 bp	1.0	70.8